Overview

• Product nameAnti-Bax抗体[6A7]
  参阅全部 Bax 一抗
• Description

  小鼠单克隆抗体[6A7] to Bax

• Tested applicationsFlow Cyt, ICC/IF, IP, WBmore details
• Species reactivity

  Reacts with: Mouse, Rat, Human, Chinese Hamster

• Immunogen

  Synthetic peptide corresponding to Human Bax aa 12-24 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
  Sequence:

  CGPTSSEQIMKTGA

  
  Database link: Q07812

  
  

  Run BLAST with Run BLAST with 

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• Storage bufferpH: 8.20
  Constituents: 0.09% Sodium borate, 0.09% Sodium chloride
  
  Sodium Borate and Sodium Chloride are used to produce a Borate-buffered saline.

• Concentration50 μg at 0.1 mg/ml 

• PurityProtein G purified
• ClonalityMonoclonal
• Clone number6A7
• IsotypeIgG1
• Research Areas
  • Cell Biology
  • 
    
  • Apoptosis
  • 
    
  • Intracellular
  • 
    
  • Associated Proteins
  • Cancer
  • 
    
  • Invasion/microenvironment
  • 
    
  • Apoptosis
  • 
    
  • Bcl 2 family
  • Metabolism
  • 
    
  • Pathways and Processes
  • 
    
  • Metabolism processes
  • 
    
  • Apoptosis

Applications

Our Abpromise guarantee covers the use of ab5714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionAccelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
• Tissue specificityExpressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
• Cellular localizationCytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
• Information by UniProt

• Database links

  • Entrez Gene: 581 Human
  • Entrez Gene: 12028 Mouse
  • Entrez Gene: 24887 Rat
  • Omim: 600040 Human
  • SwissProt: Q07812 Human
  • SwissProt: Q07813 Mouse
  • SwissProt: Q63690 Rat
  • Unigene: 624291 Human

  see all

• Alternative names

  • apoptosis regulator BAX antibody
  • Apoptosis regulator BAX cytoplasmic isoform beta antibody
  • Apoptosis regulator BAX membrane isoform alpha antibody

  see all

Anti-Bax antibody [6A7] images

• Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [6A7] (ab5714)This image is courtesy of an Abreview submitted by Dr Alwin Scharstuhl

  Immunofluorescence analysis of Human foreskin fibroblast cells, staining Bax with ab5714.

  Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin prior to being blocked in 1% BSA + 2% normal goat serum for 30 mins at 20°C. Samples were incubated with 5 μg/ml primary antibody for 45 mins at 20°C; the diution buffer was 1% BSA, 0.1% saponin, 0.05% NaN₃ in PBS. An Alexa Fluor? 594-conjugated Goat polyclonal to mouse IgG (ab150116), dilution 1/1000, was used as secondary antibody.

  See Abreview

• Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [6A7] (ab5714)This image is courtesy of an anonymous Abreview

  Immunofluorescence analysis of Human PMN cells staining Bax with ab5714.

  The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 2% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG (H+L) conjugated to Alexa Fluor? 488 (green) (ab150113) used at a 1/500 dilution. Counterstained with DAPI (blue).

  See Abreview

• Western blot - Anti-Bax antibody [6A7] (ab5714)Image courtesy of an anonymous Abreview.

  Anti-Bax antibody [6A7] (ab5714) at 1/2000 dilution + whole tissue lysate prepared from human islet tissue at 20 μg
  
  Secondary
  Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000 dilution
  developed using the ECL technique
  
  Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds

  Image courtesy of an anonymous Abreview.

  See Abreview

• Flow Cytometry-Bax antibody [6A7](ab5714)

  Overlay histogram showing HeLa cells stained with ab5714 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5714, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

Protocols

• Recommended IP protocol with ab5714
• Recommended ICC/IF protocol with ab5714

References for Anti-Bax antibody [6A7] (ab5714)

This product has been referenced in:

• Chile T  et al. HOXB7 mRNA is overexpressed in pancreatic ductal adenocarcinomas and its knockdown induces cell cycle arrest and apoptosis. BMC Cancer 13:451 (2013). Flow Cyt ; Human . Read more (PubMed: 24088503) ?
• Behensky AA  et al. Stimulation of sigma receptors with afobazole blocks activation of microglia and reduces toxicity caused by amyloid-?25-35. J Pharmacol Exp Ther 347:458-67 (2013). Read more (PubMed: 24006337) ?

See all 16 Publications for this product